CPM-5 The Cytotoxic Effects of Extract Filtration Methods on MCF-7 Breast Cancer Cell Line
Start Date
12-4-2024 9:30 AM
End Date
12-4-2024 11:30 AM
Location
University Readiness Center Greatroom
Document Type
Poster
Abstract
Mediterranean herb extracts have previously been used for various cancer treatments. The marjoram extract for this study was obtained using standard microwave extraction methods, which have been used in studies previously. However, the cytotoxic effects of various filtration methods for marjoram have not been researched in depth.
This study investigates the cytotoxic effects of filtered extracts on the MCF-7 breast cancer cell line. MCF-7 is a metastatic, hormonal-positive, HER2-negative, breast cancer cell line. The objective of this study is to determine if the filtered extracts at a lower concentration will be cytotoxic to the MCF-7 cell line when compared to the cytotoxic concentration of the primary control extract. Three filtration systems were used before exposing the extracts to the MCF-7 cells. The filtration methods used were gravity filtration using a 60C Hersch funnel with a porosity of 40-60 μm, gravity filtration using a p5 filter paper from Fisher Scientific with a porosity of 5-10 μm, and vacuum filtration using a p5 filter paper from Fisher Scientific with a porosity of 5-10 μm. The cell line was exposed to concentrations ranging from 1000 μg/mL to 1 μg/mL of the various filtered extracts and the primary control extract in respective cell media. After 48 hours, cell viability was quantified using the MTS Assay. The quantified data was analyzed using 2-sample T-tests with a Bonferroni correction.
The lowest concentration that yielded statistically significant cytotoxic effects on the MCF-7 cell line was the gravity-filtered extract using the p5 filter paper. This filtered extract may serve as a naturopathic treatment option for metastatic, hormonal-positive breast cancer.
Keywords
marjoram, filtration, cytotoxicity, MTS Assay, microwave extraction
CPM-5 The Cytotoxic Effects of Extract Filtration Methods on MCF-7 Breast Cancer Cell Line
University Readiness Center Greatroom
Mediterranean herb extracts have previously been used for various cancer treatments. The marjoram extract for this study was obtained using standard microwave extraction methods, which have been used in studies previously. However, the cytotoxic effects of various filtration methods for marjoram have not been researched in depth.
This study investigates the cytotoxic effects of filtered extracts on the MCF-7 breast cancer cell line. MCF-7 is a metastatic, hormonal-positive, HER2-negative, breast cancer cell line. The objective of this study is to determine if the filtered extracts at a lower concentration will be cytotoxic to the MCF-7 cell line when compared to the cytotoxic concentration of the primary control extract. Three filtration systems were used before exposing the extracts to the MCF-7 cells. The filtration methods used were gravity filtration using a 60C Hersch funnel with a porosity of 40-60 μm, gravity filtration using a p5 filter paper from Fisher Scientific with a porosity of 5-10 μm, and vacuum filtration using a p5 filter paper from Fisher Scientific with a porosity of 5-10 μm. The cell line was exposed to concentrations ranging from 1000 μg/mL to 1 μg/mL of the various filtered extracts and the primary control extract in respective cell media. After 48 hours, cell viability was quantified using the MTS Assay. The quantified data was analyzed using 2-sample T-tests with a Bonferroni correction.
The lowest concentration that yielded statistically significant cytotoxic effects on the MCF-7 cell line was the gravity-filtered extract using the p5 filter paper. This filtered extract may serve as a naturopathic treatment option for metastatic, hormonal-positive breast cancer.