BMB-2 High Cellular Fatty Acid Level Differentially Impacts Anaerobic Glycolytic Capacity in Human Adult and Fetal Skeletal Muscle Cells.
Abstract
Previous studies have revealed that maternal obesity increases fetal exposure to high fatty acid levels. Fetal skeletal muscle has proven to be especially sensitive to maternal nutrition. However, it remains unclear if maternal obesity can negatively impact hypoxia tolerance in fetal skeletal muscle. Hypoxia tolerance is dependent upon anaerobic glycolytic capacity. We investigated the impact of high cellular fatty acid level on glycolytic capacity in human adult and fetal skeletal muscle cells. To determine the impact of fatty acid level on cellular lipid content, fully differentiation skeletal myotubes were exposed to vehicle (BSA), 0.25, 0.5, or 1.0 mM BSA-conjugated oleic acid (OA) for 24 h prior experiments. Oil red O analysis revealed that only the highest OA dose significantly (P
Keywords
Obesity, Skeletal Muscle, Hypoxia Tolerance
BMB-2 High Cellular Fatty Acid Level Differentially Impacts Anaerobic Glycolytic Capacity in Human Adult and Fetal Skeletal Muscle Cells.
University Readiness Center Greatroom
Previous studies have revealed that maternal obesity increases fetal exposure to high fatty acid levels. Fetal skeletal muscle has proven to be especially sensitive to maternal nutrition. However, it remains unclear if maternal obesity can negatively impact hypoxia tolerance in fetal skeletal muscle. Hypoxia tolerance is dependent upon anaerobic glycolytic capacity. We investigated the impact of high cellular fatty acid level on glycolytic capacity in human adult and fetal skeletal muscle cells. To determine the impact of fatty acid level on cellular lipid content, fully differentiation skeletal myotubes were exposed to vehicle (BSA), 0.25, 0.5, or 1.0 mM BSA-conjugated oleic acid (OA) for 24 h prior experiments. Oil red O analysis revealed that only the highest OA dose significantly (P