PC-03 Peanut allergen reduction using phytic acid functionalized magnetic chitosan nanoparticles
Abstract
Peanuts (Arachis hypogea) are a nutritious plant protein that strengthens carbohydrates in foods widely consumed. Peanuts are an affordable and reliable source of proteins, vitamins, minerals, and other nutritional benefits. Although versatile, peanuts contain various proteins that are responsible for allergic reactions to predisposed individuals.
In this study, phytic acid (PA) was used to functionalize magnetic nanoparticles (MNP) that were coated with low molecular weight chitosan (CH). The synthesized MNP were exposed to extracts from three varieties of peanuts: Runner, Spanish, and Virginia. Allergen extraction was monitored by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and bicinchoninic acid (BCA) analysis. The SDS-PAGE and BCA displayed the removal of Ara h 1 and Ara h 2 allergens. BCA results shows over 69%, 44%, and 59% removed from Runner, Spanish, and Virginia, respectively. MNP-CHPA may serve as an inexpensive method for removing protein allergens in peanuts.
PC-03 Peanut allergen reduction using phytic acid functionalized magnetic chitosan nanoparticles
Peanuts (Arachis hypogea) are a nutritious plant protein that strengthens carbohydrates in foods widely consumed. Peanuts are an affordable and reliable source of proteins, vitamins, minerals, and other nutritional benefits. Although versatile, peanuts contain various proteins that are responsible for allergic reactions to predisposed individuals.
In this study, phytic acid (PA) was used to functionalize magnetic nanoparticles (MNP) that were coated with low molecular weight chitosan (CH). The synthesized MNP were exposed to extracts from three varieties of peanuts: Runner, Spanish, and Virginia. Allergen extraction was monitored by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and bicinchoninic acid (BCA) analysis. The SDS-PAGE and BCA displayed the removal of Ara h 1 and Ara h 2 allergens. BCA results shows over 69%, 44%, and 59% removed from Runner, Spanish, and Virginia, respectively. MNP-CHPA may serve as an inexpensive method for removing protein allergens in peanuts.