MC-06 Exosome Delivered MicroRNA Therapeutics for Glioblastoma
Abstract
Exosomes are small vesicles released by cells, have emerged as therapeutic vehicles for cancer treatment. Our research focuses on using exosomes enriched with microRNA(miR) cargo to restore miR dysregulation in glioblastoma (GBM), a highly aggressive form of brain cancer with a poor prognosis. Current treatment options, which are limited to surgery, radiation, and chemotherapy, have failed to improve clinical outcomes. In this study, we used exosomes isolated from human adipose stem cells (ADSCs) enriched with miR-7 and miR-124 and evaluated their effect on established GBM cell lines. Using a combination of cell viability assays, microscopy, and western blot analysis, we have assessed the impact of miR restoration and the underlying molecular mechanisms.
Our data suggested that a successful transfer of miR cargo into the target GBM cells halted cell proliferation by targeting cell proliferation pathways. The use of stem cell-derived exosomes offers several advantages such as immune system evasion and targeted delivery, making them a promising therapeutic strategy for glioblastoma.
MC-06 Exosome Delivered MicroRNA Therapeutics for Glioblastoma
Exosomes are small vesicles released by cells, have emerged as therapeutic vehicles for cancer treatment. Our research focuses on using exosomes enriched with microRNA(miR) cargo to restore miR dysregulation in glioblastoma (GBM), a highly aggressive form of brain cancer with a poor prognosis. Current treatment options, which are limited to surgery, radiation, and chemotherapy, have failed to improve clinical outcomes. In this study, we used exosomes isolated from human adipose stem cells (ADSCs) enriched with miR-7 and miR-124 and evaluated their effect on established GBM cell lines. Using a combination of cell viability assays, microscopy, and western blot analysis, we have assessed the impact of miR restoration and the underlying molecular mechanisms.
Our data suggested that a successful transfer of miR cargo into the target GBM cells halted cell proliferation by targeting cell proliferation pathways. The use of stem cell-derived exosomes offers several advantages such as immune system evasion and targeted delivery, making them a promising therapeutic strategy for glioblastoma.