PH1 -- Exposure to 17α-Ethinylestradiol Disrupts Hatch Rate and Development of Pigmentation in Zebrafish Embryos at Varied Water Temperatures
Start Date
8-4-2022 10:30 AM
End Date
8-4-2022 12:15 PM
Location
URC Greatroom
Document Type
Event
Abstract
Endocrine-disrupting chemicals (EDCs), such as17α-Ethinylestradiol (EE2), threaten to contaminate local waterways. This can be hazardous for aquatic life due to the resulting developmental changes that may ensue from exposure to these chemicals. The purpose of this study is to investigate the effect of EE2 exposure on zebrafish embryos at temperatures of 28°C and 33°C evaluating the effects of EE2 on the overall development of zebrafish via the hatch rate and rate of pigmentation expressed in zebrafish embryos. Significantly faster hatch rates and rates of pigmentation were observed for both the EE2 treatment and control groups maintained at higher temperatures compared to the groups maintained at lower temperatures. The fastest hatch rate and rate of pigmentation were observed in the 33°C control group; the rates were significantly decreased in the 33°C EE2 treatment group. The hatch rates and rates of pigmentation between the EE2 treated and control groups maintained at 28°C were very similar and were significantly slower compared to the groups raised in higher water temperatures. The data obtained suggest that increased water temperature enhances the development of zebrafish embryos and activates EE2; the activated EE2 then disrupts embryo development. The resulting disruptions to overall development observed via hatch rate and rate of pigmentation expressed may suggest changes for other markers of embryo development, such as gonad differentiation.
Keywords
Physiology
PH1 -- Exposure to 17α-Ethinylestradiol Disrupts Hatch Rate and Development of Pigmentation in Zebrafish Embryos at Varied Water Temperatures
URC Greatroom
Endocrine-disrupting chemicals (EDCs), such as17α-Ethinylestradiol (EE2), threaten to contaminate local waterways. This can be hazardous for aquatic life due to the resulting developmental changes that may ensue from exposure to these chemicals. The purpose of this study is to investigate the effect of EE2 exposure on zebrafish embryos at temperatures of 28°C and 33°C evaluating the effects of EE2 on the overall development of zebrafish via the hatch rate and rate of pigmentation expressed in zebrafish embryos. Significantly faster hatch rates and rates of pigmentation were observed for both the EE2 treatment and control groups maintained at higher temperatures compared to the groups maintained at lower temperatures. The fastest hatch rate and rate of pigmentation were observed in the 33°C control group; the rates were significantly decreased in the 33°C EE2 treatment group. The hatch rates and rates of pigmentation between the EE2 treated and control groups maintained at 28°C were very similar and were significantly slower compared to the groups raised in higher water temperatures. The data obtained suggest that increased water temperature enhances the development of zebrafish embryos and activates EE2; the activated EE2 then disrupts embryo development. The resulting disruptions to overall development observed via hatch rate and rate of pigmentation expressed may suggest changes for other markers of embryo development, such as gonad differentiation.