Date of Award
Spring 2024
Degree Type
Thesis
Department
Biological Sciences
Director of Thesis
Melissa Ellermann
First Reader
Michael Madden
Second Reader
Michael Madden
Abstract
Crohn's disease (CD) is a chronic inflammatory bowel disease. Interleukin-10 knockout (IL-10 KO) mice are crucial in CD research. Adherent-invasive Escherichia coli (AIEC) is a subgroup of E. coli. In one study, IL-10 KO mice colonized with AIEC developed colitis in the distal colon compared to wild-type (WT) mice. I investigated how AIEC NC101 WT affects colitis severity in IL-10 KO mice of the C57BL/6 genetic background. Similar results in both genotypes would enhance mouse strain accessibility. Germ-free C57BL6 IL-10 KO mice were divided into groups: colonized with AIEC NC101 for 12, 15, or 18 weeks, or kept germ-free. Histopathological analyses assessed crypt hyperplasia, abscesses, edema, and goblet cell loss in the colon segments. Cumulative scores were calculated, and a Kruskal-Wallis test revealed significantly higher scores in the middle and distal colon at 15 weeks post-colonization compared to the control. Germ-free mice showed no colitis indicators. These findings indicate AIEC's pro-inflammatory effect in IL-10 KO mice of the C57BL/6 genetic background, suggesting AIEC induces colitis in IL-10-deficient mice regardless of genetic background.
First Page
1
Last Page
27
Recommended Citation
Zaw, Sarah SM, "Adherent-Invasive Escherichia coli (AIEC) Promotes Mild Colitis in the C57BL/6 IL-10 KO Mouse Model of Crohn's Disease" (2024). Senior Theses. 669.
https://scholarcommons.sc.edu/senior_theses/669
Rights
© 2024, Sarah SM Zaw
Comments
Happy Reading future researcher!