Date of Award

Spring 2023

Degree Type



Chemistry and Biochemistry

Director of Thesis

Nicholas Truex

First Reader

Ricardo Hernandez


This study aimed to explain and adequately utilize several common biochemical laboratory techniques to mutate a portion of the nucleic acid sequence within a specific protein. This protein, referred to as Tetur02g09850 is originated from Tetranychus Urticae, or the Two-Spotted Spider Mite. This mite causes excessive damage to crops and is known to be extremely resistant to most common pesticides. For this reason, understanding the protein structure could be used to explain this acaricide resistance and aid in the development of more effective pesticides. Some of the testing utilized was site-directed mutagenesis, IMAC and gel filtration for protein purification, gel Electrophoresis, DSF, and X-ray crystallography. These experiments successfully created enhanced protein crystals which are expected to adequately diffract and provide a basis for the creation of a 3D model.

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© 2023, Hayley Cash

Included in

Biochemistry Commons