Date of Award

Spring 2023

Degree Type

Thesis

Department

Chemistry and Biochemistry

Director of Thesis

Nicholas Truex

First Reader

Ricardo Hernandez

Abstract

This study aimed to explain and adequately utilize several common biochemical laboratory techniques to mutate a portion of the nucleic acid sequence within a specific protein. This protein, referred to as Tetur02g09850 is originated from Tetranychus Urticae, or the Two-Spotted Spider Mite. This mite causes excessive damage to crops and is known to be extremely resistant to most common pesticides. For this reason, understanding the protein structure could be used to explain this acaricide resistance and aid in the development of more effective pesticides. Some of the testing utilized was site-directed mutagenesis, IMAC and gel filtration for protein purification, gel Electrophoresis, DSF, and X-ray crystallography. These experiments successfully created enhanced protein crystals which are expected to adequately diffract and provide a basis for the creation of a 3D model.

First Page

1

Last Page

21

Rights

© 2023, Hayley Cash

Download

Included in

Biochemistry Commons

Share

COinS