Date of Award
Spring 2022
Degree Type
Thesis
Department
Chemistry and Biochemistry
Director of Thesis
Dr. Qian Wang
First Reader
Jingyu Fan
Second Reader
Jingyu Fan
Abstract
Streptavidin, a bacteria-derived protein, and the small molecule biotin display extremely high-affinity binding. Researchers have taken advantage of this uniquely strong interaction in a myriad of detection, labeling, and purification techniques, one of which is affinity chromatography. Streptavidin can be immobilized onto a solid support, and this streptavidin-conjugated material can be used to capture biotin-tagged molecules with exceedingly high strength and specificity. In this project, a protocol was developed for synthesizing an agarose-based streptavidin resin for use as an affinity chromatography medium. Additionally, two fluorescence-based assays were developed for quantifying the amount of streptavidin conjugated to each resin and for quantifying the ability of each resin to capture biotin-tagged biomolecules. Several streptavidin resins were synthesized with varied reaction parameters in an attempt to maximize the resin’s ability to capture biotin-tagged molecules. The result of this project is a streptavidin resin with optimized biotin-binding capabilities that has the potential to be implemented as a versatile tool for selectively capturing biomolecules of interest.
First Page
1
Last Page
27
Recommended Citation
Thomas, Sydney, "Synthesis of a Streptavidin-Conjugated Agarose Resin for Purification of Biotin-Tagged Biomolecules" (2022). Senior Theses. 520.
https://scholarcommons.sc.edu/senior_theses/520
Rights
© 2022, Sydney Thomas