Date of Award
Spring 2019
Degree Type
Thesis
Department
Chemistry and Biochemistry
Director of Thesis
Caryn Outten
First Reader
Angela Albetel
Second Reader
Angela Albetel
Abstract
Iron dysregulation has been linked to a variety of human diseases, such as anemia, Friedreich’s ataxia, X-linked sideroblastic anemia, sideroblastic-like microcytic anemia, and myopathy. Thus, it is vitally important to understand the mechanisms for regulating intracellular iron. Here, we use fluorescence microscopy techniques in live cells to study interactions of the yeast proteins Grx3/4, Aft1/2, and Bol2, which have been shown to be involved in turning off iron import when the cell has adequate iron. Modified versions of genes encoding these proteins have been incorporated into several yeast backgrounds to use fluorescence to monitor interactions under varying iron levels.
First Page
1
Last Page
24
Recommended Citation
Rivers, William, "Characterizing Aft1/2-Grx3/4 Interaction and the Role of Bol2 During Iron Regulation in Saccharomyces cerevisiae" (2019). Senior Theses. 292.
https://scholarcommons.sc.edu/senior_theses/292
Rights
© 2019, William Rivers
Included in
Biochemical Phenomena, Metabolism, and Nutrition Commons, Biochemistry Commons, Fungi Commons, Laboratory and Basic Science Research Commons, Molecular Biology Commons, Nutritional and Metabolic Diseases Commons