Event Title

MB5 -- Does miR-718 affect PLC-B2 expression and CREB phosphorylation?

Location

URC Greatroom

Start Date

8-4-2022 10:30 AM

End Date

8-4-2022 12:15 PM

Description

Autism spectrum disorder (ASD) manifestations in behavior include aggressive tendencies, issues with social communication and other challenging behaviors. The number of diagnoses has increased over the past decade. There are also some dysregulated cellular mechanisms, seen in patients with ASD that effect brain morphology. For example, cell cleaning, or autophagy, has been shown to be decreased in ASD brains, and autophagy dysregulation can result in increased dendritic spines in the brain. Further, Calcium signaling and neurotransmission are lost to some degree in ASD patients. MicroRNAs are small RNAs, roughly 22 nucleotides long, that can bind to and degrade many different messenger RNAs, and they can exert their effects on many different pathways. Previously in our lab, miR-718 was shown to have effects on autophagy through targeting PTEN. MiR-718 is also suspected to target PLC-B2, which is an enzyme that cleaves phosphatidylinositol molecules in the cell membrane and form two second messengers: IP3 and DAG. IP3 binds the smooth ER membrane to allow for Calcium release, which affects transcription factor CREB’s phosphorylation and subsequently gene expression. Here, we present data for our investigation of whether miR-718 targets PLC-B2 and whether this subsequently affects downstream CREB phosphorylation.

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Apr 8th, 10:30 AM Apr 8th, 12:15 PM

MB5 -- Does miR-718 affect PLC-B2 expression and CREB phosphorylation?

URC Greatroom

Autism spectrum disorder (ASD) manifestations in behavior include aggressive tendencies, issues with social communication and other challenging behaviors. The number of diagnoses has increased over the past decade. There are also some dysregulated cellular mechanisms, seen in patients with ASD that effect brain morphology. For example, cell cleaning, or autophagy, has been shown to be decreased in ASD brains, and autophagy dysregulation can result in increased dendritic spines in the brain. Further, Calcium signaling and neurotransmission are lost to some degree in ASD patients. MicroRNAs are small RNAs, roughly 22 nucleotides long, that can bind to and degrade many different messenger RNAs, and they can exert their effects on many different pathways. Previously in our lab, miR-718 was shown to have effects on autophagy through targeting PTEN. MiR-718 is also suspected to target PLC-B2, which is an enzyme that cleaves phosphatidylinositol molecules in the cell membrane and form two second messengers: IP3 and DAG. IP3 binds the smooth ER membrane to allow for Calcium release, which affects transcription factor CREB’s phosphorylation and subsequently gene expression. Here, we present data for our investigation of whether miR-718 targets PLC-B2 and whether this subsequently affects downstream CREB phosphorylation.