Document Type
Article
Abstract
Background
The Zanzibar archipelago has been a pre-elimination region for malaria thanks to rigorous control interventions, but recent surges in malaria cases have been observed. The contribution of non-falciparum species to the current malaria situation is unknown.
Method
This study investigates the epidemiology of falciparum and non-falciparum malaria species in Zanzibar. Leveraging plasma extracted from dried blood spots (DBS) collected during reactive case detection (RCD) activities across Unguja island from May 2022 to January 2023, we measured immunoglobulin G (IgG) responses to Plasmodium MSP1-19kD antigens using a multiplex bead-based immunoassay. Additionally, active infections were detected using species-specific real-time PCR.
Results
Out of 1618 participants surveyed in 35 RCDs, 35.3% had exposure to any malaria species, with Plasmodium falciparum being the most common (29.8%). Seroprevalences for non-falciparum species were lower: Plasmodium ovale (5.8%), Plasmodium malariae (5.9%), and Plasmodium vivax (5.9%). Active infections were detected in 6.0% of participants, predominantly P. falciparum (4.6%). Travel to mainland Tanzania was a dominant risk factor for seropositivity for all 4 malaria species. Other factors associated with Pf seropositivity (high-risk occupations and female status) were not associated with seropositivity for non-falciparum species. The geographic distribution of non-falciparum exposure differed compared to falciparum, with relatively higher seroprevalences in rural districts, especially Kazkazini A in northern Unguja.
Conclusions
This study suggests a significant contribution of non-falciparum species to the local epidemiology in Zanzibar. Current control and elimination efforts, focused on P. falciparum, may not adequately address exposure to non-falciparum species.
Digital Object Identifier (DOI)
Publication Info
Published in Open Forum Infectious Diseases, Volume 13, Issue 2, 2026, pages ofag051-.
Rights
© 2026, © The Author(s) 2026. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This is an open access article distributed under the terms of the Creative Commons CC BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
APA Citation
Goel, V., Hassan, W., Murphy, C., Choloi, B. B., Ali, M., Mohamed, B., Zacharia, A., Dominick, M. C., Thwai, K., Mohammed, S., Shija, S. J., Bailey, J. A., Björkman, A., Ngasala, B. E., Rogier, E., Juliano, J. J., & Lin, J. T. (2026). Understanding the Epidemiology of Malaria in Zanzibar Through Molecular and Serological Analysis of Samples Collected During Reactive Case Detection. Open Forum Infectious Diseases, 13(2).https://doi.org/10.1093/ofid/ofag051