Date of Award

Summer 2023

Document Type

Open Access Thesis

Department

Biomedical Science

First Advisor

Frank Spinale

Abstract

While echocardiography continues to be a renowned imaging approach to study LV function and geometry in mice, contrast enhanced micro-CT has recently gained popularity because of the high-resolution three-dimensional images produced. However, comparative analyses between echocardiography and micro-CT are limited and conflicting. Therefore, to demonstrate the validity of micro-CT against echocardiography, this study aimed to compare identical measurements of LV function and geometry in wildtype and genetically modified mice in which confounding parameters such as time and anesthetic conditions were taken into consideration. Globally deleted FAP knockout mice (n=12) and age-matched wild type mice (n=14) were included in this study. Contrast enhanced micro-CT and echocardiography were performed three days apart, monitoring for anesthetic depth and duration. Following cardiac gating qualification,10 mice (WT: n=7, FAPKO: n=3) were removed from the data set due to high quantities of raw projections with ventricular displacements in the micro-CT reconstructions. Across all groups, ESV and HR measured by micro-CT were significantly higher than values obtained by echocardiography, while SV, EF, CO and LV mass measured by micro-CT were significantly lower. Micro-CT detected an increase in LV mass within FAPKO mice compared to WT mice (100.3 ± 3.8, 83.2 ± 4.5 mg, respectively, p

Rights

© 2023, Kyle Porter Stegmann

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