Date of Award


Document Type

Open Access Thesis


Biomedical Science


School of Medicine

First Advisor

Robert Price


The goal of this thesis is to systematically examine each decision made in obtaining images on the Leica SP8 Multiphoton Confocal Microscope and discuss the basic operating parameters in order to generate publication quality images. The capabilities of the LAS X software are discussed along with an in depth discussion of each icon in order, beginning with the Configuration icon, moving then to Acquire, and a brief overview of the possible imaging processing functions within the Process icon. At the conclusion of this thesis, the reader should feel comfortable with the operation of a confocal system and understand the tradeoffs that occur between obtaining high quality images, specimen damage, and the time it takes to collect the image. Although this paper discusses the operation of the Leica SP8 Multiphoton Confocal Microscope specifically, the principles of confocal microscopy hold true regardless of the system hardware or software used.


© 2018, Amy E. Rowley