Date of Award
Open Access Dissertation
The widespread influence of miRNAs in many biological processes and characteristic changes in expression profiles make them potential biomarkers for a range of diseases. With recent discoveries highlighting the enrichment of particular circulating miRNAs in plasma under pathological conditions, there has been a push for technological advances in miRNA profiling studies as a diagnostic tool. A standardized, automated, high-throughput method for miRNA isolation from human plasma samples was developed in this study. Samples were subject to full miRNome profiling utilizing RT-qPCR. These unique findings demonstrate that developing a distinct miRNA biomarker profile for heart failure patients is feasible and holds potential diagnostic value.
N. Zellars, K.(2017). Advancing Automated Methods for Microrna Profiling. (Doctoral dissertation). Retrieved from https://scholarcommons.sc.edu/etd/4421