Date of Award


Document Type

Open Access Dissertation


Biological Sciences

First Advisor

Francis Spinale


The widespread influence of miRNAs in many biological processes and characteristic changes in expression profiles make them potential biomarkers for a range of diseases. With recent discoveries highlighting the enrichment of particular circulating miRNAs in plasma under pathological conditions, there has been a push for technological advances in miRNA profiling studies as a diagnostic tool. A standardized, automated, high-throughput method for miRNA isolation from human plasma samples was developed in this study. Samples were subject to full miRNome profiling utilizing RT-qPCR. These unique findings demonstrate that developing a distinct miRNA biomarker profile for heart failure patients is feasible and holds potential diagnostic value.