Document Type
Article
Subject Area(s)
Biomarkers; Disease Susceptibility (immunology); Gene Expression Profiling; Humans; Immunity, Innate; Immunity, Mucosal; Immunophenotyping; Inflammatory Bowel Diseases (etiology, metabolism, pathology); Interleukin-10 (genetics, metabolism); Intestinal Mucosa (immunology, metabolism, pathology); Macrophages (immunology, metabolism); Membrane Glycoproteins (genetics, metabolism); Receptors, Immunologic (genetics, metabolism); Transcriptome
Abstract
To understand the contribution of mononuclear phagocytes (MNP), which include monocyte-derived intestinal macrophages, to the pathogenesis of inflammatory bowel disease (IBD), it is necessary to identify functionally-different MNP populations. We aimed to characterise intestinal macrophage populations in patients with IBD. We developed 12-parameter flow cytometry protocols to identify and human intestinal MNPs. We used these protocols to purify and characterize colonic macrophages from colonic tissue from patients with Crohn's disease (CD), ulcerative colitis (UC), or non-inflamed controls, in a cross-sectional study. We identify macrophage populations (CD45CD64 HLA-DR) and describe two distinct subsets, differentiated by their expression of the mannose receptor, CD206. CD206 macrophages expressed markers consistent with a mature phenotype: high levels of CD68 and CD163, higher transcription of IL-10 and lower expression of TREM1. CD206 macrophages appear to be less mature, with features more similar to their monocytic precursors. We identified and purified macrophage populations from human colon. These appear to be derived from a monocytic precursor with high CCR2 and low CD206 expression. As these cells mature, they acquire expression of IL-10, CD206, CD63, and CD168. Targeting the newly recruited monocyte-derived cells may represent a fruitful avenue to ameliorate chronic inflammation in IBD.
Digital Object Identifier (DOI)
Publication Info
Published in Scientific Reports, Volume 11, 2021, pages 19616-.
APA Citation
Wright, P. B., McDonald, E., Bravo-Blas, A., Baer, H. M., Heawood, A., Bain, C. C., Mowat, A. M., Clay, S. L., Robertson, E. V., Morton, F., Nijjar, J. S., Ijaz, U. Z., Milling, S. W. F., & Gaya, D. R. (2021). The mannose receptor (CD206) identifies a population of colonic macrophages in health and inflammatory bowel disease. Scientific Reports, 11, 19616. https://doi.org/10.1038/s41598-021-98611-7
Rights
© The Author(s) 2021 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.