UCL23 Hierarchically Regulated by WRKY51-miR528 Mediates Cadmium Uptake, Tolerance, and Accumulation in Rice
Document Type
Article
Subject Area(s)
Oryza (metabolism, genetics); Cadmium (metabolism, toxicity); MicroRNAs (metabolism, genetics); Plant Proteins (metabolism, genetics); Gene Expression Regulation, Plant; Transcription Factors (metabolism, genetics); Plant Roots (metabolism); Promoter Regions, Genetic
Abstract
In humans, cadmium (Cd) toxicity caused by contaminated environments is associated with numerous chronic diseases. Breeding rice with low Cd accumulation is now deemed critical for sustainable agriculture development. Here, we elucidate the crucial functions of UCLACYANIN 23 (UCL23), a small copper protein, in Cd absorption, tolerance, and accumulation through modulation of reactive oxygen signals in rice. Additionally, we demonstrate that WRKY51 binds to promoters of UCL23 and miR528, a post-transcriptional regulator of UCL23, thereby contributing to Cd regulation in a dual-modulatory manner. Furthermore, we show that the natural variation of UCL23 is important for the differential accumulation of Cd in rice grains. Finally, we reveal that Indica rice harboring the major Japonica haplotype of UCL23 significantly reduces Cd uptake in roots and Cd accumulation in grains. Together, our study not only reveals a regulatory cascade in Cd regulation but also provides valuable resources for breeding low-Cd rice cultivars.
Digital Object Identifier (DOI)
Publication Info
Published in Cell Reports, Volume 44, Issue 3, 2025, pages 115336-.
APA Citation
Tan, J., Zhang, L., Liu, C., Hong, Z., Wu, X., Zhang, Y., Fahad, M., Shen, Y., Bian, J., He, H., Wu, D., Shu, Q., Bao, J., & Wu, L. (2025). UCL23 hierarchically regulated by WRKY51-miR528 mediates cadmium uptake, tolerance, and accumulation in rice. Cell Reports, 44(3), 115336.https://doi.org/10.1016/j.celrep.2025.115336
Rights
© 2025 The Author(s). Published by Elsevier Inc. This is an open access article distributed under the terms of the Creative Commons CC-BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.