The HKT Transporter HvHKT1;5 Negatively Regulates Salt Tolerance
Document Type
Article
Abstract
Maintaining low intracellular Na concentrations is an essential physiological strategy in salt stress tolerance in most cereal crops. Here, we characterized a member of the high-affinity K transporter (HKT) family in barley (Hordeum vulgare), HvHKT1;5, which negatively regulates salt tolerance and has different functions from its homology in other cereal crops. HvHKT1;5 encodes a plasma membrane protein localized to root stele cells, particularly in xylem parenchyma cells adjacent to the xylem vessels. Its expression was highly induced by salt stress. Heterogenous expression of HvHKT1;5 in Xenopus laevis oocytes showed that HvHKT1;5 was permeable to Na, but not to K, although its Na transport activity was inhibited by external K HvHKT1;5 knockdown barley lines showed improved salt tolerance, a dramatic decrease in Na translocation from roots to shoots, and increases in K/Na when compared with wild-type plants under salt stress. The negative regulation of HvHKT1;5 in salt tolerance distinguishes it from other HKT1;5 members, indicating that barley has a distinct Na transport system. These findings provide a deeper understanding of the functions of HKT family members and the regulation of HvHKT1;5 in improving salt tolerance of barley.
Digital Object Identifier (DOI)
Publication Info
Published in Plant Physiology, Volume 182, Issue 1, 2020, pages 584-596.
APA Citation
Huang, L., Kuang, L., Wu, L., Shen, Q., Han, Y., Jiang, L., Wu, D., & Zhang, G. (2019). The HKT Transporter HvHKT1;5 Negatively Regulates Salt Tolerance. Plant Physiology, 182(1), 584–596.https://doi.org/10.1104/pp.19.00882
Rights
© 2020 American Society of Plant Biologists. All Rights Reserved.