Document Type
Article
Abstract
Male sterility is widely used in the production of hybrid seeds in rice, but the use of genic male sterility is limited because of the high labour cost for maintaining male-sterile lines. Previous studies using T-DNA insertional mutagenesis demonstrated that disrupting the expression of oxophytodienoic acid reductase 3 (OPR3), which is involved in the jasmonate biosynthesis pathway, results in a kind of male sterility that can be restored to fertility by exogenous jasmonate in Arabidopsis. Here, we created male-sterile mutations by editing the second and fourth exons of OsOPR7 in rice through clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system 9. The induced mutagenesis at these exons resulted in 31.8% and 23.9% male-sterile plants in the T generation, respectively. We screened male-sterile lines that can be restored to fertility by exogenous methyl jasmonate in the T T and T2 rice populations and characterized the anther and agronomic traits of the transgenic plants. Results showed the successful generation of male-sterile lines through the silencing of OsOPR7, the orthologous gene of Arabidopsis OPR3, in a field crop, paving the way for the establishment of a two-line system for rice hybrid production. The system consists of a male-sterile line that can be maintained by spraying methyl jasmonate and a restoring line that confers pollen.
Digital Object Identifier (DOI)
Publication Info
Plant Biotechnology Journal, Volume 19, Issue 2, 2021, pages 365-374.
APA Citation
Pak, H., Wang, H., Kim, Y., Song, U., Tu, M., Wu, D., & Jiang, L. (2020). Creation of male‐sterile lines that can be restored to fertility by exogenous methyl jasmonate for the establishment of a two‐line system for the hybrid production of rice ( Oryza sativa L.). Plant Biotechnology Journal, 19(2), 365–374. https://doi.org/10.1111/pbi.13471
Rights
© 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.