https://doi.org/10.1016/j.jhazmat.2021.127140

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Transcriptome-Wide M6A Methylation Profile Reveals Regulatory Networks in Roots of Barley Under Cadmium Stress

Document Type

Article

Abstract

Cadmium (Cd) pollutants restrict crop yield and food security in long-term agricultural activities. Crops have evolved adaptive strategies under Cd condition, however, the transcriptional regulatory mechanism of Cd-tolerant genes remains to be largely illustrated. In this study, barley roots were exposed to 5 µM CdCl for physiological response and transcriptome-wide m6A methylation profile. Cd stress inhibited root growth after 7 d Cd treatment, which is mainly associated with inhibited absorption of Mn. After Cd treatment, 8151 significantly modified m6A sites and 3920 differentially expressed genes were identified. Transcriptome-wide m6A hypermethylation was widely induced by Cd stress and enriched near the stop codon and 3' UTR regions. Among 435 m6A modified DEGs, 319 hypermethylated genes were up-regulated and 84 hypomethylated genes were down-regulated, respectively, indicating a positive correlation of m6A methylation and expression. But well-known Cd transporter genes (HvNramp5, HvIRT1, HvHMA3, etc.) were not modified by m6A methylation, except for ABC transporters. We further found key Cd-responding regulatory genes were positively modulated with m6A methylation, including MAPK, WRKY and MYB members. This study proposed a transcriptional regulatory network of Cd stress response in barley roots, which may provide new insight into gene manipulation of controlling low Cd accumulation for crops.

Digital Object Identifier (DOI)

https://doi.org/10.1016/j.jhazmat.2021.127140

APA Citation

Su, T., Fu, L., Kuang, L., Chen, D., Zhang, G., Shen, Q., & Wu, D. (2022). Transcriptome-wide m6A methylation profile reveals regulatory networks in roots of barley under cadmium stress. Journal of Hazardous Materials,/i>, 423(Part A), 127140.https://doi.org/10.1016/j.jhazmat.2021.127140

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© 2021 Elsevier B.V. All rights reserved.

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