Document Type
Article
Abstract
Brownian motion of Cowpea mosaic virus (CPMV) in water was measured using small-angle X-ray photon correlation spectroscopy (SA-XPCS) at 19.2 mu s time resolution. It was found that the decorrelation time tau(Q) = 1/DQ(2) up to Q = 0.091 nm(-1). The hydrodynamic radius R-H determined from XPCS using Stokes-Einstein diffusion D = kT/(6 pi eta R-H) is 43% larger than the geometric radius R-0 determined from SAXS in the 0.007 M K3PO4 buffer solution, whereas it is 80% larger for CPMV in 0.5 M NaCl and 104% larger in 0.5 M (NH4)(2)SO4, a possible effect of aggregation as well as slight variation of the structures of the capsid resulting from the salt-protein interactions.
Digital Object Identifier (DOI)
Publication Info
Published in Journal of Synchrotron Radiation, Volume 29, 2022, pages 1429-1435.
Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
APA Citation
Switalski, K., Fan, J., Li, L., Chu, M., Sarnello, E., Jemian, P. R., Li, T., Wang, Q., & Zhang, Q. (2022). Direct measurement of Stokes–Einstein diffusion of Cowpea mosaic virus with 19 µs-resolved XPCS. Journal of Synchrotron Radiation, 29(6), 1429–1435. https://doi.org/10.1107/S1600577522008402