Document Type
Article
Subject Area(s)
Materials Science, Biology
Abstract
Thymidylate synthase (TS) is a well validated target in cancer chemotherapy. Here, a new crystal form of the R163K variant of human TS (hTS) with five subunits per asymmetric part of the unit cell, all with loop 181-197 in the active conformation, is reported. This form allows binding studies by soaking crystals in artificial mother liquors containing ligands that bind in the active site. Using this approach, crystal structures of hTS complexes with FdUMP and dUMP were obtained, indicating that this form should facilitate high-throughput analysis of hTS complexes with drug candidates. Crystal soaking experiments using oxidized glutathione revealed that hTS binds this ligand. Interestingly, the two types of binding observed are both asymmetric. In one subunit of the physiological dimer covalent modification of the catalytic nucleophile Cys195 takes place, while in another dimer a noncovalent adduct with reduced glutathione is formed in one of the active sites.
Digital Object Identifier (DOI)
https://doi.org/10.1107/S0907444910044732
Publication Info
Published in Acta Crystallographica Section D: Biological Crystallography, Volume 67, Issue 1, 2010, pages 60-66.
Rights
© Acta Crystallographica Section D: Biological Crystallography 2011, International Union of Crystallography.
APA Citation
Gibson, L., Celeste, L., Lovelace, L., & Lebioda, L. (2010). Structures of Human Thymidylate Synthase R163K with dUMP, FdUMP and Glutathione Show Asymmetric Ligand Binding. Acta Crystallographica Section D: Biological Crystallography, 67(1), 60–66. https://doi.org/10.1107/S0907444910044732