The Effects of Super-Resolution Microscopy on Colocalization Conclusions Previously Made With Diffraction-Limited Systems in the Biomedical Sciences
Date of Award
Open Access Thesis
Super-resolution microscopy, also known as nanoscopy, is now established in the field of microscopy. The evolution of super-resolution microscopy techniques has allowed many scientists to peer past the most significant limitation in conventional fluorescence microscopy – the diffraction limit. Super-resolution microscopy systems are able to resolve two points that are less than 250 nm apart, which supersedes the resolution of diffraction limited systems. Colocalization studies are frequently used in the biomedical sciences to measure the close physical association and functional interaction of two biological structures. Previous colocalization conclusions made with conventional fluorescence microscopy are largely based on how the colors of two fluorophores such as a red fluorophore and a green fluorophore combine to create a yellow color. However, qualitative conclusions based on standard resolution instruments need to be critically reviewed now that super-resolution microscopy techniques supersede the diffraction limit and scientists can now evaluate structures and molecules that are laterally and axially less than 250 nm apart.
Yemc, M. E.(2020). The Effects of Super-Resolution Microscopy on Colocalization Conclusions Previously Made With Diffraction-Limited Systems in the Biomedical Sciences. (Master's thesis). Retrieved from https://scholarcommons.sc.edu/etd/6115