An Unbiased Approach to Identify Genes Involved in Development in a Turtle with Temperature-Dependent Sex Determination
Many reptiles exhibit temperature-dependent sex determination (TSD). The initial cue in TSD is incubation temperature, unlike genotypic sex determination (GSD) where it is determined by the presence of specific alleles (or genetic loci). We used patterns of gene expression to identify candidates for genes with a role in TSD and other developmental processes without making a priori assumptions about the identity of these genes (ortholog-based approach). We identified genes with sexually dimorphic mRNA accumulation during the temperature sensitive period of development in the Red-eared slider turtle (Trachemys scripta), a turtle with TSD. Genes with differential mRNA accumulation in response to estrogen (estradiol-17β; E(2)) exposure and developmental stages were also identified.
Published in BMC Genomics, Volume 13, Issue 1, 2012, pages 308-319.
© 2012 Chojnowski and Braun; licensee BioMed Central Ltd.
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