Dielectrophoretic Separation of Colorectal Cancer Cells

Author(s)

F. Yang
Xiaoming Yang, University of South Carolina - ColumbiaFollow
H. Jiang
P. Bulkhaults
Patricia Wood, University of South Carolina - ColumbiaFollow
William Hrushesky, University of South Carolina - ColumbiaFollow
Guiren Wang, University of South Carolina - ColumbiaFollow

Document Type

Article

Subject Area(s)

Fluid Dynamics, Plasma and Beam Physics

Abstract

Separation of colorectal cancercells from other biological materials is important for stool-based diagnosis of colorectal cancer. In this paper, we use conventional dielectrophoresis in a microfluidic chip to manipulate and isolate HCT116 colorectal cancercells. It is noticed that at a particular alternating current frequency band, the HCT116 cells are clearly deflected to a side channel from the main channel after the electric activation of an electrode pair. This motion caused by negative dielectrophoresis can be used to simply and rapidly separate cancercells from other cells. In this manuscript, we report the chip design, flow conditions, dielectrophoretic spectrum of the cancercells, and the enrichment factor of the colorectal cancercells from other cells.

Publication Info

Published in Biomicrofluidics, Volume 4, Issue 1, 2010, pages 013204-.

Rights

Copyright 2010 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics.

The following article appeared in

Yang, F., Yang, X., Jiang, H., Bulkhaults, P., Wood, P., Hrushesky, W., & Wang, G. (2010). Dielectrophoretic separation of colorectal cancer cells. Biomicrofluidics, 4(1), 013204. http://dx.doi.org/10.1063/1.3279786

and may be found at

http://scitation.aip.org/content/aip/journal/bmf/4/1/10.1063/1.3279786