Document Type

Article

Subject Area(s)

Computer Science and Engineering, Chemistry

Abstract

At pH > 6 added filamentous bacteriophage fd is compatible with many of the detergents used to solubilize membrane proteins for solution NMR studies of membrane proteins and, therefore, serves as an alignment media. In combination with strained polyacrylamide gel alignment, Dipolar Waves can be used to directly assess the secondary structure and a λ-map extracts the order tensors for de novo structure calculation of membrane proteins without distance restraints.

Rights

http://pubs.acs.org/journal/jacsat

© 2009 by the American Chemical Society

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