Event Title

CB6 -- Peanut allergen isolation using ligand bound magnetic nanoparticles

Location

URC Greatroom

Start Date

8-4-2022 10:30 AM

End Date

8-4-2022 12:15 PM

Description

Protein (Arachis hypogaea) has a balanced and high nutrient composition of fats, proteins, carbohydrates, vitamins and minerals, and it is widely consumed around the globe due to its affordability. The major concern with peanut consumption, however, is the allergenic reactions of some of its protein constituents (Ara h1-17). In the U.S., 2.5% of children and 1.8% of adults have some range of mild to severe peanut allergenicity, which can only be treated through avoidance of consumption or possible immunotherapy. The goal of this study was to develop a cheap, simple, and recyclable extraction technology for allergens removal from peanut extracts. In this study, we functionalized magnetic iron oxide nanoparticles (M) with phytic acid (PA) and evaluated the potential application of the coated particles as an adsorbent for removing allergens from extracts of two varieties of peanuts, the Spanish and the Valencia. The ligand bound magnetic iron oxide nanoparticles (MPA) were characterized using spectroscopic methods. Protein concentrations and protein profiling of the peanut extracts were carried out by bicinchoninic acid (BCA) protein assay and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis, respectively. The SDS-PAGE profiles show near complete removal of Ara h1 in the raw and defatted extracts of the two varieties studied after 19 h treatment with MPA (7.9 ± 0.050 mg MPA/mL extract). Ara h1 is the most abundant peanut allergen recognized by the serum IgE in more than 90% of peanut-allergic patients. The MPA are inexpensive, easy to use, easy to retrieve after use, and a prospective adsorbent for highly efficient removal of Ara h1 from raw and defatted peanut extract. The desorption of adsorbed Ara h1 from MPA for allergy research and therapy processes will be discussed.

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Apr 8th, 10:30 AM Apr 8th, 12:15 PM

CB6 -- Peanut allergen isolation using ligand bound magnetic nanoparticles

URC Greatroom

Protein (Arachis hypogaea) has a balanced and high nutrient composition of fats, proteins, carbohydrates, vitamins and minerals, and it is widely consumed around the globe due to its affordability. The major concern with peanut consumption, however, is the allergenic reactions of some of its protein constituents (Ara h1-17). In the U.S., 2.5% of children and 1.8% of adults have some range of mild to severe peanut allergenicity, which can only be treated through avoidance of consumption or possible immunotherapy. The goal of this study was to develop a cheap, simple, and recyclable extraction technology for allergens removal from peanut extracts. In this study, we functionalized magnetic iron oxide nanoparticles (M) with phytic acid (PA) and evaluated the potential application of the coated particles as an adsorbent for removing allergens from extracts of two varieties of peanuts, the Spanish and the Valencia. The ligand bound magnetic iron oxide nanoparticles (MPA) were characterized using spectroscopic methods. Protein concentrations and protein profiling of the peanut extracts were carried out by bicinchoninic acid (BCA) protein assay and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis, respectively. The SDS-PAGE profiles show near complete removal of Ara h1 in the raw and defatted extracts of the two varieties studied after 19 h treatment with MPA (7.9 ± 0.050 mg MPA/mL extract). Ara h1 is the most abundant peanut allergen recognized by the serum IgE in more than 90% of peanut-allergic patients. The MPA are inexpensive, easy to use, easy to retrieve after use, and a prospective adsorbent for highly efficient removal of Ara h1 from raw and defatted peanut extract. The desorption of adsorbed Ara h1 from MPA for allergy research and therapy processes will be discussed.