Dielectrophoretic Separation of Colorectal Cancer Cells
Fluid Dynamics, Plasma and Beam Physics
Separation of colorectal cancercells from other biological materials is important for stool-based diagnosis of colorectal cancer. In this paper, we use conventional dielectrophoresis in a microfluidic chip to manipulate and isolate HCT116 colorectal cancercells. It is noticed that at a particular alternating current frequency band, the HCT116 cells are clearly deflected to a side channel from the main channel after the electric activation of an electrode pair. This motion caused by negative dielectrophoresis can be used to simply and rapidly separate cancercells from other cells. In this manuscript, we report the chip design, flow conditions, dielectrophoretic spectrum of the cancercells, and the enrichment factor of the colorectal cancercells from other cells.
Published in Biomicrofluidics, Volume 4, Issue 1, 2010, pages 013204-.
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The following article appeared in
Yang, F., Yang, X., Jiang, H., Bulkhaults, P., Wood, P., Hrushesky, W., & Wang, G. (2010). Dielectrophoretic separation of colorectal cancer cells. Biomicrofluidics, 4(1), 013204. http://dx.doi.org/10.1063/1.3279786
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