Date of Award
Open Access Thesis
Environmental Health Sciences
Norman J. Arnold School of Public Health
Vibrio gazogenes, a Gram-negative species of marine bacteria, was capable of transporting 20 nm (diameter) surface-carboxylated, polystyrene, fluorescent FluoSpheres® microspheres (excitation/emission = 505/515) through the outer membrane, which was indicated by a detectable decrease in the fluorescence intensity of the nanoparticles in the culture medium. A mechanism of transport was investigated involving the ATP-binding cassette (ABC) protein transporters that traverse the outer membrane. Inhibition of the ABC transporters did not prevent the entry of the nanoparticles into the cell, suggesting there was an alternate mechanism of transport. The addition of nanoparticles to the culture medium also did not provide any growth benefits for Vibrio gazogenes.
Jones, S. R.(2016). Uptake of Nanoparticles by Vibrio Gazogenes. (Master's thesis). Retrieved from http://scholarcommons.sc.edu/etd/3606